Illuminate what
matters
Clarity. Speed. Confidence.
You bring the samples and ideas. We bring multiplex IF reagents to reveal the biological information you need – spatial biology at the ease of standard IHC.
You bring the samples and ideas. We bring multiplex IF reagents to reveal the biological information you need – spatial biology at the ease of standard IHC.
What matters
to you?
You know your biomarkers. Your science. Your questions. We’re here to make sure the complexity of spatial biology doesn’t slow you down.
Revolune delivers a reagent system designed to remove friction - so you can focus on discovery, not on decoding protocols.
Revolune delivers a reagent system designed to remove friction - so you can focus on discovery, not on decoding protocols.
Revolune reveals what matters to you.
Tumor. Microenvironment. Tissue. Biomarkers. Phenotyping. Answers. Context.
Clarity
Matters
Bright staining for clear answers.
You select the targets you want to locate in your tissue. They should stand out clearly over background and autofluorescence.
We deliver reagents to strongly amplify signals that matter to you. High signal to noise ratio and large dynamic range facilitate automated quantification across multiple markers in a full tissue context.
You select the targets you want to locate in your tissue. They should stand out clearly over background and autofluorescence.
We deliver reagents to strongly amplify signals that matter to you. High signal to noise ratio and large dynamic range facilitate automated quantification across multiple markers in a full tissue context.
Speed
Matters
Simple assays development. Short time to results. Elegantly simple protocol.
You can mix and match your established antibodies to build mIF panels quickly. Straightforward assay development helps you to start your experiment sooner. Short run times get you results faster.
We thought hard about our reagents, so you won’t have to. We reduced the steps in our protocol and the moving parts in our chemistry to reduce complexity and run time. Protocols can be run on autostainers or manually, while fluorophores are compatible with many imaging systems.
You can mix and match your established antibodies to build mIF panels quickly. Straightforward assay development helps you to start your experiment sooner. Short run times get you results faster.
We thought hard about our reagents, so you won’t have to. We reduced the steps in our protocol and the moving parts in our chemistry to reduce complexity and run time. Protocols can be run on autostainers or manually, while fluorophores are compatible with many imaging systems.
Confidence
Matters
Conclusive data for your discoveries. Future-proof for your diagnostic journey.
You need solid data and results that you can trust to make your decisions. You need reagents that scale with your projects and are ready for regulated environments if required.
We built our reagents to accelerate your discovery. We tightly control the components of our reagent system to ensure consistency and robustness. We can provide and scale IVD-ready reagents according to ISO13485 when you need them.
You need solid data and results that you can trust to make your decisions. You need reagents that scale with your projects and are ready for regulated environments if required.
We built our reagents to accelerate your discovery. We tightly control the components of our reagent system to ensure consistency and robustness. We can provide and scale IVD-ready reagents according to ISO13485 when you need them.
Focus on
your science
Don't worry about the workflow, instead focus on your science.
No antibody stripping. No harsh treatments.
Protect every tissue section.
No conjugation.
Use the antibodies you already trust. Continue to use panels you already established.
No rigid detection rules.
Mix and match primary antibodies. Pair antibodies and fluorophores however you choose.
No lock-in. No specialized hardware.
Run the protocol on automated platforms or manually. Image with the instruments you already have.
Fast, flexible assay development using the antibodies you already trust. Think about the targets you want to see. We have thought about everything else. Our reagents enable 5-plex assays including nuclear stain today. We will release additions to enable 8-plex assays soon.
WORKFLOW
01
01
Add your antibodies in one step
Use your existing, pre-validated primary antibodies without modification. Simply pre-incubate each antibody with one of our Connectors. Mix your pre-incubated primary antibody-Connector complexes and apply them to the tissue section in a single step.
- Use your trusted antibodies – no conjugation or revalidation
- Adjust panels easily
- Reduce staining time and complexity

02
02
Amplify your signal
Our amplification reagents build a DNA scaffold at each of our Connectors.
- Detect low abundance targets
- Increase signal without adding background noise
- Get more data from every tissue section

03
03
Detect what matters to you
Fluorescent probes bind to multiple sites on each DNA scaffold. Therefore, many fluorophores are recruited to each antibody site to yield bright staining.
- Produce bright signals for confident analysis
- Work seamlessly with standard fluorescent imaging systems
- Deliver consistent, reproducible results across experiments


Ultimately, what matters to you is a workflow you can trust.
One that removes the friction, failure points, and complexity that have long burdened spatial biology.One that protects your tissue, accelerates your assays, reduces your costs, and gives you the clarity to answer the scientific questions that matter most.
One that removes the friction, failure points, and complexity that have long burdened spatial biology.One that protects your tissue, accelerates your assays, reduces your costs, and gives you the clarity to answer the scientific questions that matter most.
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We keep you
Focused on
What Matters
We make your biomarkers shine. Our workflow removes the friction, failure points, and complexity that have long burdened spatial biology. It protects your tissue, accelerates your assay development, reduces your costs, and gives you the clarity to answer the scientific questions that matter most.
We built this workflow to deliver Clarity, Speed, and Confidence.
We built this workflow to deliver Clarity, Speed, and Confidence.

Revolune reveals what matters to you.
Scalability. Flexibility. Compatibility. Multiplex. Profiling. Signatures. Answers. Context.
Be the first
Tech access program
Join Revolune’s early access program and experience multiplex immunofluorescence with the simplicity of standard IHC.
At Revolune, we’re rethinking how spatial biology gets done - making high-quality multiplexing accessible, efficient, and compatible with how you already work. As part of our Illuminate First early access program, we’re inviting innovative labs to test-drive our reagent platform. You bring your samples and your go-to primary antibodies - we’ll provide the secondary reagents to reveal deeper biological insights. No specialized setup. No complex protocols. Just clean, fast, multiplexed results.
Be first. Be bold. Apply now.
Be first. Be bold. Apply now.
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