Illuminate what matters with out-of-the-box multiplex IF
High-performance multiplex immunofluorescence reagents designed to simplify assay development, preserve tissue integrity, and generate bright, reproducible signals without the complexity of traditional workflows.
Multiplex IF, without the usual trade-offs
Traditional multiplex IF workflows often introduce unnecessary complexity. Illuminate Reagents remove those barriers, enabling fast, flexible multiplexing without stripping, conjugation, staining-sequence optimization, or specialized imaging requirements.
Protect every tissue section
No antibody stripping. No harsh treatment cycles. Parallel staining and amplification help preserve tissue integrity and reduce failure risk across precious sample cohorts.
Use the antibodies you trust
No antibody conjugation required.
Start with your existing primary antibodies and build panels with less revalidation burden.*
* Initial offering supports rabbit IgG antibodies.
Illuminate What Matters
Build panels faster
Skip staining-sequence optimization and simplify assay development from the start. Illuminate Reagents are designed to make multiplex IF faster, easier, and more repeatable.
Stay flexible from assay to image
Mix and match detection signals, adapt biomarker panels, and image with standard fluorescence microscopy systems. Built for flexibility — not lock-in.
How Illuminate Reagents work
A simple three-step approach designed to make multiplex IF faster, gentler, and easier to adopt. It protects your tissue, accelerates your assay development, reduces your costs, and gives you the clarity to answer the scientific questions that matter most.
Add your antibodies in one step
Use your existing, pre-validated primary antibodies without modification. Simply pre-incubate each antibody with one of our Connectors. Mix your pre-incubated primary antibody-Connector complexes and apply them to the tissue section in a single step.
- Use your trusted antibodies – no conjugation or revalidation
- Adjust panels easily
- Reduce staining time and complexity
Amplify your signal
Our amplification reagents build a DNA scaffold at each of our Connectors.
- Detect low abundance targets
- Increase signal without adding background noise
- Get more data from every tissue section
Detect what matters to you
Fluorescent probes bind to multiple sites on each DNA scaffold. Therefore, many fluorophores are recruited to each antibody site to yield bright staining.
- Produce bright signals for confident analysis
- Work seamlessly with standard fluorescent imaging systems
- Deliver consistent, reproducible results across experiments
Revolune Illuminate-P4-R
A modular reagent system for flexible multiplex IF assay development. This kit is designed to perform single or multiplex immunofluorescence stainings of FFPE (Formalin-Fixed Paraffin-Embedded) samples.
Details
The user can select up to four different rabbit IgG primary antibodies (Ab). These Ab are pre-labeled with 4 different Connectors (488, 555, 647 & 750). The staining protocol can be performed manually or on the Leica Bond system. The reagents are sufficient to stain 10 slides.
Multiplex IF Kits for Every Stage of Discovery
From focused biomarker studies to higher-plex spatial profiling, the Illuminate Reagent portfolio scales with your research needs. Choose from 4-plex to 7-plex configurations and generate high-quality multiplex IF data using the antibodies, workflows, and imaging systems you already trust.
Works within your existing ecosystem
Compatible with standard staining workflows
Run manually or adapt to automated staining platforms for scalable, reproducible assay execution.
Instrument-agnostic imaging
Image on standard fluorescence microscopy systems without being locked into a dedicated imaging platform.
Ready for downstream analysis
Generate multiplex IF images suitable for quantitative spatial analysis, AI-powered workflows, and translational research applications.
Built for adoption
Transfer existing assay knowledge into multiplex IF with less workflow disruption, fewer new dependencies, and faster implementation.
Want the technical details?
Download our manual and automation-ready protocols to see how easily Illuminate Reagents can fit into your multiplex IF workflow.