Want the technical details?
Download our manual and automation-ready protocols to see how easily Illuminate Reagents can fit into your multiplex IF workflow.
Add your antibodies in one step
Use your existing, pre-validated primary antibodies without modification. Simply pre-incubate each antibody with one of our Connectors. Mix your pre-incubated primary antibody-Connector complexes and apply them to the tissue section in a single step.
- Use your trusted antibodies – no conjugation or revalidation
- Adjust panels easily
- Reduce staining time and complexity
Amplify your signal
Our amplification reagents build a DNA scaffold at each of our Connectors.
- Detect low abundance targets
- Increase signal without adding background noise
- Get more data from every tissue section
Detect what matters to you
Fluorescent probes bind to multiple sites on each DNA scaffold. Therefore, many fluorophores are recruited to each antibody site to yield bright staining.
- Produce bright signals for confident analysis
- Work seamlessly with standard fluorescent imaging systems
- Deliver consistent, reproducible results across experiments
Revolune Illuminate-P4-R
A modular reagent system for flexible multiplex IF assay development. This kit is designed to perform single or multiplex immunofluorescence stainings of FFPE (Formalin-Fixed Paraffin-Embedded) samples.
Details
The user can select up to four different rabbit IgG primary antibodies (Ab). These Ab are pre-labeled with 4 different Connectors (488, 555, 647 & 750). The staining protocol can be performed manually or on the Leica Bond system. The reagents are sufficient to stain 10 slides.
Multiplex IF Kits for Every Stage of Discovery
From focused biomarker studies to higher-plex spatial profiling, the Illuminate Reagent portfolio scales with your research needs. Choose from 4-plex to 7-plex configurations and generate high-quality multiplex IF data using the antibodies, workflows, and imaging systems you already trust.
